Use of Molecular Methods for the Rapid Mass Detection of Schistosoma mansoni (Platyhelminthes: Trematoda) in Biomphalaria spp. (Gastropoda: Planorbidae)

Figure 5

Fluorescence of LAMP products from molluscs pool using SYBR Green I staining followed by ultraviolet illumination at 320 nm. Tubes 1 and 2: infected Biomphalaria glabrata in prepatent period: 1 positive snail in a pool of 20 negative snails and 1 : 300; Tubes from 3 to 6: infected B. glabrata shedding cercariae: 1 : 20; 1 : 300; 1 : 500; and 1 : 1000; Tube 7: infected Biomphalaria straminea in prepatent period: 1 : 100; Tube 8: infected B. straminea shedding cercariae; Tube 9: negative control.