Efficiency of the Stool-PCR Test Targeting NADH Dehydrogenase (Nad) Subunits for Detection of Opisthorchis viverrini Eggs

Figure 3

PCR amplicons of OvNad subunits using the new introduced primers and its cross-reactivity evaluation. (a) OvNad1, (b) OvNad2, (c) OvNad4, and (d) OvNad5 (M, 100 bp plus DNA marker; NC, no template control, PC; positive control from uncountable Ov-eggs dissected from adult O. viverrini; and 1 to 7 are the samples processed by PECT, 1, O. viverrini egg detected sample; 2, parasite undetected sample; 3, hookworm egg detected sample; 4, Ascaris lumbricoides egg detected sample; 5, Taenia spp. egg detected sample; 6, Strongyloides stercoralis larva detected sample; and 7, Trichuris trichiura egg detected sample.

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