Development of Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick Assay for a Rapid and Sensitive Detection of Cystic Echinococcosis in Livestock in Kenya

Figure 2

Detection of the LAMP products; the reaction was conducted at 63°C for 40 minutes. (a) Determination of the LAMP turbidity by a real-time turbidimeter. (b) 3% agarose gel electrophoresis of nested PCR products. (c) Visual detection using SYBR Green 1 dye under visible light and UV light. (d) LAMP-LFD detection: CL and TL indicate control line and test line, respectively. Lines 1–3 represent positive samples; PC is positive control and NC is negative control. Lane M: 1 kb DNA molecular marker.

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