Development of Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick Assay for a Rapid and Sensitive Detection of Cystic Echinococcosis in Livestock in Kenya

Figure 4

Analytical sensitivity of LAMP and nested PCR products using 10-fold dilutions of E. granulosus DNA at 63°C for 40 minutes. (a) LAMP products detected by LFD strips; CL indicates control line and TL indicates test line for positive samples. (b) Visual detection of LAMP products using SYBR Green 1 dye under visible (green) and UV light (bright fluorescence), respectively. (c) Real-time LAMP turbidity monitored by a Loopamp real-time turbidimeter at 400 nm. The amplification curve graph was generated by GraphPad Prism software version 8.00. (d) 3% agarose gel electrophoresis of LAMP products. (e) Nested PCR products analysis on 1.5% agarose gel; M: DNA molecular marker; N: no template control; 1–7: 10-fold serial dilution of E. granulosus DNA from 10⁻¹ to 10⁻⁷ (1 ng to 1 fg).

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