Research Article
The Comparison of PCR Kits for the Detection of Erythrocytic Parasites on Filter Paper
Table 2
DBS PCR results of 20 DNA polymerases.
| | DNA polymerases | Whatman CF12 (parasites/µL) | Advantec 545 (parasites/µL) | Gel Blot paper (parasites/µL) | Jesiman Filter Faper (parasites/µL) | Positive rates of 20 polymerases | | 101 | 102 | 103 | 101 | 102 | 103 | 101 | 102 | 103 | 101 | 102 | 103 |
| | Beyotime | HemoTaq | ＋ | ＋ | ＋ | − | ＋ | ＋ | ＋ | ＋ | ＋ | − | ＋ | ＋ | 83.30% (10/12) | | Taq | − | ＋ | ＋ | − | ＋ | ＋ | − | − | ＋ | − | − | ＋ | 50.00% (6/12) | | HemoTaq HF | − | − | − | − | − | − | − | − | − | − | − | ＋ | 8.30% (1/12) | | BeyoFusion | − | − | − | − | − | − | − | − | − | − | − | − | 0.00% (0/12) |
| | Sangon biotech | Direct PCR kit | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | 100.00% (12/12) | | Taq Plus | − | − | − | − | − | − | − | − | − | − | − | − | 0.00% (0/12) |
| | TAKARA | Tks Gflex | ＋ | ＋ | ＋ | − | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | 91.70% (11/12) | | Taq | ＋ | ＋ | ＋ | − | ＋ | ＋ | − | ＋ | ＋ | ＋ | ＋ | ＋ | 83.30% (10/12) | | MightyAmp | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | 100.00% (12/12) |
| | TOYOBO | rTaq | − | − | ＋ | − | − | ＋ | − | − | ＋ | − | ＋ | ＋ | 41.70% (5/12) | | KOD Plus Neo | − | ＋ | ＋ | − | ＋ | ＋ | − | ＋ | ＋ | ＋ | ＋ | ＋ | 75.00% (9/12) | | KOD FX Neo | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | 100.00% (12/12) |
| | TIANGEN | Mouse Tissue Direct PCR kit | − | ＋ | ＋ | ＋ | ＋ | ＋ | − | ＋ | ＋ | ＋ | ＋ | ＋ | 83.30% (10/12) | | Ultra HiFidelity | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | 100.00% (12/12) | | FOREVERSTAR | Blood direct PCR kit | − | − | − | − | − | − | − | − | − | − | − | − | 0.00% (0/12) |
| | Thermo Fisher | Phire Hot Start II | − | ＋ | ＋ | − | ＋ | ＋ | − | − | ＋ | − | ＋ | ＋ | 58.30% (7/12) | | Platinum direct PCR kit | − | ＋ | ＋ | − | ＋ | ＋ | − | ＋ | ＋ | − | − | ＋ | 58.30% (7/12) |
| | NEB | Hemo klen Taq | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | 100.00% (12/12) | | Vent | − | − | − | − | − | − | − | − | − | − | − | − | 0.00% (0/12) | | Q5 | ＋ | ＋ | ＋ | ＋ | ＋ | ＋ | − | ＋ | ＋ | − | ＋ | ＋ | 83.30% (10/12) |
| | Positive rates of 4 filter papers | 45.00% (9/20) | 70.00% (14/20) | 75.00% (15/20) | 35.00% (7/20) | 70.00% (14/20) | 75.00% (15/20) | 35.00% (7/20) | 60.00% (12/20) | 75.00% (15/20) | 45.00% (9/20) | 65.00% (13/20) | 80.00% (16/20) | | | 63.30% (38/60) | 60.00% (36/60) | 56.70% (34/60) | 63.30% (38/60) | |
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