The Comparison of PCR Kits for the Detection of Erythrocytic Parasites on Filter Paper
Table 3
Cost-effectiveness comparison of 20 DNA polymerases.
DNA polymerases
Item no.
Price (USD)
Tests
Price/test (USD)
Positive rate (n/12) (%)
Scores
Scores (price/test)
NEB Hemo klen Taq
M0332S
186.55
200
0.93
100.00
444
476.00
TAKARA MightyAmp
R071Q
40.95
40
1.02
100.00
444
433.69
Ultra HiFidelity PCR kit
KP203
97.28
80
1.22
100.00
444
365.14
TOYOBO KOD FX Neo
KFX-201S
31.38
20
1.57
100.00
444
282.98
Sangon Direct PCR kit
B639289-0050
82.37
50
1.65
100.00
444
269.51
TAKARA Tks Gflex
R060Q
75.47
80
0.94
100.00
344
364.65
TAKARA Taq
R001 A
18.83
200
0.09
83.30
244
2,591.88
Beyotime HemoTaq
D7241S
39.70
200
0.20
83.30
244
1,229.35
Mouse Tissue Direct PCR kit
KG205
62.76
50
1.26
83.30
244
194.39
NEB Q5
M0491S
137.92
100
1.38
83.30
244
176.92
TOYOBO KOD Plus Neo
KOD-401S
23.54
20
1.18
75.00
144
122.37
Phire Hot Start II
F122S
220.76
200
1.10
58.30
34
30.80
Platinum Direct PCR kit
A44647100
136.97
100
1.37
58.30
34
24.82
Beyotime Beyotime Taq
D7205
4.39
160
0.03
50.00
24
874.08
TOYOBO rTaq
TAP-211
23.54
100
0.24
41.70
14
59.49
Beyotime HemoTaq HF
D7243S
47.85
200
0.24
8.30
1
4.18
Beyotime BeyoFusion
D7220
16.95
80
0.21
0.00
0
0.00
Sangon Taq Plus
B500013-0100
3.45
20
0.17
0.00
0
0.00
StarLighter Blood Direct PCR
FS-P6001
18.83
500
0.04
0.00
0
0.00
NEB Vent
M0254 V
61.03
200
0.31
0.00
0
0.00
Scores were calculated based on the PCR results of 20 polymerases: 1 point for positive of 103 parasites, 10 points for 102 parasites, and 100 points for 101 parasites.
