Isolation, Identification, and Genetic Characterization of Antibiotic Resistance of Salmonella Species Isolated from Chicken Farms

Table 1

Oligonucleotide primer sequences and their corresponding genes are used for the detection of antimicrobial-resistant genes in Salmonella isolates.

Gene	Primer sequence (5′-3′)	Length of amplified product	Annealing temperature (°C)	Reference
mphA	GTG​AGG​AGG​AGC​TTC​GCG​AG	403 bp	58	[34]
	TGC​CGC​AGG​ACT​CGG​AGG​TC
ereA	GCC​GGT​GCT​CAT​GAA​CTT​GAG	420 bp	60
	CGA​CTC​TAT​TCG​ATC​AGA​GGC
ermB	GAA​AAA​GTA​CTC​AAC​CAA​ATA	639 bp	45
	AATTTAAGTACCGTTACT
bla TEM	ATCAGCAATAAACCAGC	516 bp	54	[35]
	CCCCGAAGAACGTTTTC
bla SHV	AGG​ATT​GAC​TGC​CTT​TTT​G	392 bp	54
	ATTTGCTGATTTCGCTCG
ompC F	ATC​GCT​GAC​TTA​TGC​AAT​CG	204 bp	57	[33]
ompC R	CGG​GTT​GCG​TTA​TAG​GTC​TG	204 bp