|
| Gene | Size | Polymerase chain reaction protocol | Sequence of gene |
|
| UidA | 147 bp | Initial denaturation was carried out for 7 min at 95°C followed by total 35 cycles of 30 sec each at 95°C; annealing for 30 sec at 55°C; extension for 30 sec at 72°C, followed by final extension at 72°C, for 7 min | F-TGGTAATTACCGAC GAAAACGGC
R-ACGCGTG GTTACAGTCTTGCG |
|
| blaCTX-M-15 | 996 bp | Initial denaturation for 4 min at 96°C followed by total 34 cycles of 20 sec each at 95°C; annealing for 20 sec at 56°C; 60 sec at 72°C, followed by final extension at 72°C, for 5 min | F-CACACGTGGAATTTAGGGACT
R-GCCGTCTAAGGCGATAAACA |
|
| tetB | 634 bp | Initial denaturation for 3 min at 95°C followed by total 35 cycles of 1 min each at 94°C; annealing for 90 sec at 56°C; 60 sec at 72°C, followed by final extension at 72°C, for 8 min | F-CCTCAGCTTCTCAACGCGTG
R-GCACCTTGCTGATGACTCTT |
|
| TMP-SMX dfrA1 | 367 bp | Initial denaturation for 3 min at 95°C followed by total 35 cycles of 1 min each at 94°C; annealing for 90 sec at 45°C; 60 sec at 72°C, followed by final extension at 72°C, for 8 min | F-GGAGTGCCAAAGGTGAACAGC
R-GAGGCGAAGTCTTGGGTAAAAAC |
|
| PAP | | Initial denaturing 3 min at 94°C, 35 cycles of 1 min at 94°C, 1 min at 58°C, 1 min at 72°C followed by final extension of 5 min at 72°C | F-AACCTGGCTTACGCAACTGTACCC GT
R-CTG CAA AAT CAT GGA T |
|
| Heat-labile toxin | | Initial denaturing 3 min at 94°C, 35 cycles of 1 min at 94°C, 1 min at 58°C, and 1 min at 72°C and final extension for 5 min at 72°C | F-GCACACGGAGCTCCTCAGTC
R-TCCTTCATCCTT TCA ATG GCT TT |
|
