Efficiency of Automated Viral RNA Purification for Pediatric Studies of Dengue and Zika in Hyperendemic Areas

Figure 2

Characteristics of RNA isolated automatically. (a) Absolute concentration of RNA automatically purified from the plasma of pediatric patients with and without confirmed DENV infection. (b) Relative purity of the samples analyzed by the λ ratio 260/280. λ260/λ280 nm: ratio of the maximum absorbance obtained at the wavelengths (λ) of 260 and 280 nm. The detection limit of the NanoDrop™ 2000 = 1.3 ng/µL is shown as a dotted line. The value obtained with the Mann‒Whitney test is shown for each case. (c) Electrophoresis in 2% agarose gel of the total RNA purified automatically from plasma using the standard protocol (upper) and the high-throughput protocol (lower). The results of 20 pediatric samples are shown.

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