Efficiency of Automated Viral RNA Purification for Pediatric Studies of Dengue and Zika in Hyperendemic Areas

Figure 3

Amplification by RT-qPCR of the RNase P gene in automatically purified RNA by the standard protocol (a) and the high-throughput protocol (b). The cycle threshold (C_t) value was previously established at ΔRn 1 × 10⁵ by prior validations. The arrows blue and red show the amplification curves of the RNase P gene in positive and negative control, respectively. Results of 20 pediatric plasma samples added to positive and negative control (cell culture supernatant with or without DENV infection) are shown.