Efficiency of Automated Viral RNA Purification for Pediatric Studies of Dengue and Zika in Hyperendemic Areas

Table 2

Summary of results of detection of DENV obtained from purified RNA isolated automated with standard and high-throughput protocols.

Code	Silica column-based isolation; QIAGEN	Automated purification KingFisher^TM Flex		Result
		Standard	High-throughput
	RT-PCR^a	RT-qPCR^b
SSH 12	DENV-2	DENV-2	DENV-2	Matched
SSH 16	DENV-1	DENV-1	DENV-1	Matched
SSH 21	DENV-2	DENV-2	DENV-2	Matched
SSH 27	DENV-2	DENV-2	DENV-2	Matched
SSH 35	NEG	NEG	NEG	Matched
SSH 38	DENV-2	DENV-2	NEG	Mismatched
SSH 42	DENV-2	DENV-2	DENV-2	Matched
SSH 44	NEG	NEG	NEG	Matched
SSH 68	DENV-1	DENV-1	DENV-1	Matched
SSH 75	NEG	DENV-1	DENV-1	Mismatched
SSH 80	DENV-1	DENV-1	NEG	Mismatched
SSH 105	NEG	NEG	NEG	Matched
SSH 107	DENV-1	DENV-1	DENV-1	Matched
SSH 112	NEG	NEG	NEG	Matched
SSH 114	DENV-1	DENV-1	DENV-1	Matched
SSH 119	NEG	NEG	NEG	Matched
SSH 120	DENV-1	DENV-1	DENV-1	Matched
SSH 128	NEG	NEG	NEG	Matched
SSH 130	NEG	NEG	NEG	Matched
SSH 146	DENV-1	DENV-1	DENV-1	Matched
Control + ^c	DENV-1, DENV-2	DENV-1, DENV-2	DENV-1, DENV-2	Matched
Mock^d	NEG	NEG	NEG	Matched

^aConventional nested RT-PCR was used as a gold standard¹³. ^bSerotype-specific RT-qPCR used to detect DENV-1 and DENV-2. ^cMix of supernatants of Vero-76 cell culture infected with DENV-1 and DENV-2. ^dSupernatants of Vero-76 cells uninfected with DENV.