Journal of Tropical Medicine

Research Article

Deglycosylation of Excretory-Secretory Antigens of the Second-Stage Larvae of Toxocara cati Improves Its Efficacy in the Diagnosis of Human Toxocariasis

Table 1

Results of ELISA and western blotting using Toxocara excretory-secretory (TES) and deglycosylated Toxocara excretory-secretory (dTES) antigens on 30 confirmed cases of toxocariasis, 30 patients with other diseases, and 30 healthy individuals for the serodiagnosis of human toxocariasis.


Samples		Toxocariasis	HI	Other diseases
Samples		Toxocariasis	HI	A	S	Tri	H	F	VL	T	M	FUO
No. of cases		30	30	3	4	3	4	4	3	3	3	3

TES ELISA	+	29 (96.7%)	6 (20%)	1 (33.3%)	1 (25%)	1 (33.3%)	0 (0%)	2 (50%)	1 (33.3%)	0 (0%)	0 (0%)	0 (0%)
TES ELISA	−	1 (3.3%)	24 (80%)	2 (66.7%)	3 (75%)	2 (66.7%)	4 (100%)	2 (50%)	2 (66.7%)	3 (100%)	3 (100%)	3 (100%)

dTES ELISA	+	28 (93.3%)	3 (10%)	1 (33.3%)	1 (25%)	1 (33.3%)	0 (0%)	1 (25%)	0 (0%)	0 (0%)	0 (0%)	0 (0%)
dTES ELISA	−	2 (6.7%)	27 (90%)	2 (66.7%)	3 (75%)	2 (66.7%)	4 (100%)	3 (75%)	3 (100%)	3 (100%)	3 (100%)	3 (100%)

TES western blotting	+	30 (100%)	0 (0%)	1 (33.3%)	0 (0%)	1 (33.3%)	0 (0%)	1 (33.3%)	0 (0%)	0 (0%)	0 (0%)	0 (0%)
TES western blotting	−	0 (0%)	30 (100%)	2 (66.7%)	4 (100%)	2 (66.7%)	4 (100%)	3 (66.7%)	3 (100%)	3 (100%)	3 (100%)	3 (100%)

dTES western blotting	+	30 (100%)	0 (0%)	1 (33.3%)	0 (0%)	0 (0%)	0 (0%)	0 (0%)	0 (0%)	0 (0%)	0 (0%)	0 (0%)
dTES western blotting	−	0 (0%)	30 (100%)	2 (66.7%)	4 (100%)	3 (100%)	4 (100%)	4 (100%)	3 (100%)	3 (100%)	3 (100%)	3 (100%)

HI = healthy individuals, A = ascariasis, S = strongyloidiasis, Tri = trichostrongylosis, H = hydatid cyst, F = fascioliasis, VL = visceral leishmaniasis, T = toxoplasmosis, M = malaria, FUO = fever of unknown origin.