Terpenoids and Bio-Functions of Essential Oils Hydrodistilled Differently from Freshly Immature and Mature Blumea balsamifera Leaves

Table 4

Quantitative analysis of camphor, L-borneol, and ß-caryophyllene in essential oils from immature and mature leaves of B. balsamifera with different hydrodistillation periods.

Treatment		(μg^1/100 g FW)
Maturity	Time	Camphor	L-borneol	ß-caryophyllene
Immature		147.89 ± 261.24^a	58.72 ± 104.95^a	22.18 ± 35.35^a
Mature		140.12 ± 242.80^a	45.34 ± 77.08^a	19.02 ± 29.11^b
F test (maturity)		ns	ns
	0–6 hrs	559.13 ± 63.44^a	199.40 ± 52.28^a	73.80 ± 8.60^a
	6–12 hrs	14.43 ± 3.19^b	7.12 ± 1.16^b	6.31 ± 0.76^b
	12–18 hrs	1.88 ± 0.63^b	1.15 ± 0.14^b	1.61 ± 0.27^c
	18–24 hrs	0.57 ± 0.24^b	0.44 ± 0.04^b	0.68 ± 0.15^c
F test (time)
(Maturity × time)		ns	ns
Immature	0–6 hrs	576.11 ± 91.83^a	225.87 ± 68.22^a	80.48 ± 6.87^a
	6–12 hrs	13.35 ± 3.30^b	7.44 ± 1.60^c	6.06 ± 0.42^cd
	12–18 hrs	1.66 ± 0.79^b	1.16 ± 0.22^c	1.58 ± 0.31^de
	18–24 hrs	0.42 ± 0.16^b	0.43 ± 0.02^c	0.59 ± 0.12^e
Mature	0–6 hrs	542.16 ± 27.64^a	172.93 ± 8.87^b	67.11 ± 1.92^b
	6–12 hrs	15.50 ± 3.33^b	6.81 ± 0.74^c	6.55 ± 1.04^c
	12–18 hrs	2.11 ± 0.46^b	1.13 ± 0.04^c	1.64 ± 0.29^de
	18–24 hrs	0.72 ± 0.22^b	0.46 ± 0.05^c	0.76 ± 0.14^e
F-test
C.V. (%)		23.57	46.76	12.42

Data are expressed as mean ± SD (n = 3) (¹calculated by comparing to the authentic standard). Values in the same column followed by different letters indicate significant differences at  < 0.05 according to Duncan’s multiple range test; ns: not significant and : significant at  < 0.01 level.