Research Article
Distinct Allelic Diversity of Plasmodium vivax Merozoite Surface Protein 3-Alpha (PvMSP-3α) Gene in Thailand Using PCR-RFLP
Table 2
Allelic subtype frequencies of PvMSP-3α gene determined by PCR-RFLP.
| | Province | Hha I RFLP | Alu I RFLP | Combination patterns of Hha I and Alu I RFLP | | Allelic subtype | n | % | Allelic subtype | n | % | Allelic subtype | n | % |
| | Yala (southern Thailand) (n = 78) | A1 | 6 | 5.1 | A1 | 1 | 0.8 | HA1 | 2 | 1.7 | | A2 | 5 | 4.2 | A2 | 11 | 9.3 | HA2 | 1 | 0.8 | | A10 | 4 | 3.4 | A3 | 5 | 4.2 | HA3 | 2 | 1.7 | | A12 | 12 | 10.2 | A5 | 4 | 3.4 | HA11 | 2 | 0.8 | | A13 | 23 | 19.5 | | | | HA12 | 2 | 1.7 | | | | | | | | HA15 | 3 | 2.5 | | | | | | | | HA16 | 4 | 3.4 | | | | | | | | HA17 | 4 | 3.4 | | Mixed type | 28 | 23.7 | Mixed type | 57 | 48.3 | Mixed type | 57 | 48.3 |
| | Kanchanaburi (southern Thailand) (n = 18) | A1 | 4 | 3.4 | A2 | 7 | 5.9 | HA1 | 1 | 0.8 | | A4 | 1 | 0.8 | A5 | 6 | 5.1 | HA3 | 3 | 2.5 | | A6 | 4 | 3.4 | | | | HA5 | 1 | 0.8 | | A7 | 1 | 0.8 | | | | HA8 | 4 | 3.4 | | A11 | 1 | 0.8 | | | | HA13 | 1 | 0.8 | | A14 | 1 | 0.8 | | | | HA19 | 1 | 0.8 | | A15 | 2 | 1.7 | | | | HA20 | 1 | 0.8 | | A16 | 1 | 0.8 | | | | HA21 | 1 | 0.8 | | Mixed type | 3 | 2.5 | Mixed type | 5 | 4.2 | Mixed type | 5 | 4.2 |
| | Mae Hong Son (western Thailand) (n = 14) | A3 | 1 | 0.8 | A1 | 1 | 0.8 | HA4 | 1 | 0.8 | | A5 | 2 | 1.7 | A2 | 4 | 3.4 | HA6 | 1 | 0.8 | | A8 | 3 | 2.5 | A4 | 1 | 0.8 | HA7 | 1 | 0.8 | | A9 | 1 | 0.8 | A5 | 2 | 1.7 | HA9 | 2 | 1.7 | | A13 | 1 | 0.8 | | | | HA10 | 1 | 0.8 | | B3 | 1 | 0.8 | | | | HA18 | 1 | 0.8 | | Mixed type | 5 | 4.2 | Mixed type | 6 | 5.1 | Mixed type | 7 | 5.9 |
| | Tak (western Thailand) (n = 8) | B1 | 2 | 1.7 | C1 | 1 | 0.8 | — | — | — | | B2 | 1 | 0.8 | | | | | | | | B3 | 1 | 0.8 | | | | | | | | Mixed type | 4 | 3.4 | Mixed type | 7 | 5.9 | Mixed type | 7 | 6.8 |
| | Total (N = 118) | A1–A16 | 73 | 61.0 | A1–A5 | 42 | 35.6 | HA1-HA22 | 41 | 34.7 | | B1–B3 | 5 | 4.2 | C1 | 1 | 0.8 | | Mixed type | 40 | 33.9 | Mixed type | 75 | 63.6 | Mixed type | 77 | 65.3 |
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The mixed genotypes were designated as mixed types.
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