Research Article

A Novel Multiplex LAMP Assay for the Rapid and Accurate Diagnosis of Visceral Leishmaniasis Caused by Leishmania infantum from Iran

Figure 2

Nested PCR of L. major (M), L. tropica (T), and L. infantum (IN) on the left side of the ladder (L) were loaded in 2% gel agarose electrophoresis. Nested PCR detected 4 × 106 plasmid copy number/μL of L. infantum and L. tropica and detected 4 × 105 plasmid copy number/μL of L. major.