A Novel Multiplex LAMP Assay for the Rapid and Accurate Diagnosis of Visceral Leishmaniasis Caused by Leishmania infantum from Iran

Figure 3

Properties of serial dilution microtubes of plasmid cloned with ITS2 region; Overall multiplex LAMP and real-time PCR were more sensitive than nested PCR for L. infantum, L. major, and L. tropica. On the account of L. major and L. tropica, multiplex LAMP, and real-time PCR depicted almost equal sensitivity but about L. infantum, multiplex LAMP was more sensitive than real-time PCR.