A Novel Multiplex LAMP Assay for the Rapid and Accurate Diagnosis of Visceral Leishmaniasis Caused by Leishmania infantum from Iran

Table 2

PCR amplification of the ITS2 region of Leishmania was amplified by T100 thermal cycler (Bio-Rad, USA).

Temperature (°C)	Time	Steps
95	5 min	Early denaturation
95	30 sec	Denaturation	Cycling steps ≠ 30
49	30 sec	Annealing
72	90 sec	Extension
72	10 min	Final extension