A Novel Multiplex LAMP Assay for the Rapid and Accurate Diagnosis of Visceral Leishmaniasis Caused by Leishmania infantum from Iran

Table 3

Nested external and internal primers.

Gene	TM	Primer	Sequence	Product size (bp)
ITS1, ITS2, 28S (external)	57.3	Forward	AGG​CGT​GTG​TTT​GTG​TTG​TG	439 bp
	58.24	Reverse	AGAGTGAGGGCGCGGATA
ITS1, ITS2, 28S (internal)	59.35	Forward	AAC​TCC​TCT​CTG​GTG​CTT​GC	189 bp
	55.25	Reverse	AAA​ATG​GCC​AAC​GCG​AAG​TT