A Novel Multiplex LAMP Assay for the Rapid and Accurate Diagnosis of Visceral Leishmaniasis Caused by Leishmania infantum from Iran

Table 5

Two sets of LAMP1, 2 primers designed for multiplex LAMP.

Gene	TM	Primer	Sequence
ITS2 LAMP SET1	54.66	F3	TTC​TCT​TTT​TCT​CTC​TCC​ATT​C
	52.35	B3	ACA​CAC​ACA​ACC​TAC​TTC​T
	75.16	FIP	TCC​TGG​TCA​CAG​CCT​CTC​TCT​CCT​CTC​TTT​TTT​CAT​CAA​AAA​GG
	73.45	BIP	AAC​GAG​AAT​TCA​ACT​TCG​CGT​TGA​CAC​AGA​GAG​AGA​GCC​AC
ITS2 LAMP SET2	52.01	F3	ACC​AAA​ACG​AGA​ATT​CAA​CTT
	53.20	B3	TCT​TTT​TTC​TCT​GTG​CGT​AC
	73.55	FIP	TAC​CAC​ACA​GAG​AGA​GAG​CCA​CCG​TTG​GCC​ATT​TTT​TGC​T
	73.25	BIP	TAG​AAG​TAG​GTT​GTG​TGT​GTG​TAT​GTA​TGA​GAG​AGT​GAG​GGC​G