Peripheral Regulatory Cells Immunophenotyping in Kidney Transplant Recipients with Different Clinical Profiles: A Cross-Sectional Study

Figure 2

Percentage of immunoglobulin expression on IL-10-producing B peripheral cell subtype in KTR. (a) Autofluorescence control. (b) PE-conjugated-anti-rat-IL-10 IgG isotype control. (c–e) IgG₁-FITC/IgG₁-PE/CD45-PeCy5 mouse IgG₁,k isotype controls. (f) Representative contour plot of CD19⁺ B cells from a patient. An electronic gate was made for CD38^hi cells. (g) From the gate 𝑓 CD19⁺/CD38^hi/IgM⁺ cells were determined. From the latter CD19⁺/CD38^hi/IgM⁺/IL-10⁺, cells were defined in (h) a healthy donor (HD), (i) a chronic graft dysfunction (CGD) patient, and (j) an excellent long-term graft function under immunosuppression (ELTGF) patient. A total of 30,000–50,000 events were recorded for each sample before any gate setting and analyzed with the CellQuest Pro software (BD Biosciences). Bar graphs show percentage of (k) IgA⁺, (l) IgD⁺, (m) IgG⁺, and (n) CD19⁺/CD38^hi/ IL-10⁺/IgM⁺ cells. Results are expressed as median, 10th, 25th, 75th, and 90th percentiles.

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