Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum Albumin
Figure 1
Distention of pancreas with collagenase solution. (a) Before injection of collagenase, a bulldog clamp (silver bar at bottom left) is placed around the duodenum (Du) and over the Papilla of Vater of the common bile duct to prevent drainage into the duodenum. The common bile duct (BD) is cannulated with angled hypodermic needle (upper left of picture) in preparation to suffuse pancreas with collagenase. The pancreas is the whitish mass between the spleen (Sp), stomach (St), liver, and intestines. (b) Same picture after inflation. A green vegetable dye is used here to visualize full distension of the pancreas.
