Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum Albumin

Figure 3

Perifusion assay after 24 hours in culture. A dynamic perifusion assay for insulin response was performed with 75 islets in each group. After a 30 minute preincubation step, islets were exposed for 30 minutes to low glucose (2.8 mM) then for 30 minutes to high glucose (20 mM) and back to 30 minutes of low glucose. Circle shapes represent the BSA group and square shapes represent the group with No BSA. Graph shown was one of 9 assays performed. There was no significant difference between the 2 groups for insulin response to glucose at 24 hours.