Research Article
Noninvasive Evaluation of EGFR Expression of Digestive Tumors Using 99mTc-MAG3-Cet-F(ab)2-Based SPECT/CT Imaging
Figure 3
Verification of Cet-F(ab)2 on its molecular weight, binding affinity to EGFR positive cells, and in vitro stability. (a) SDS-PAGE showing that the molecular weight of Cet-F(ab)2 and cetuximab is 100 kDa and 150 kDa, respectively. (b) Immunocytochemistry showing high binding ability of Cet-F(ab)2 and cetuximab to MGC803 cells. (c) SDS-PAGE illustrating the excellent stability of Cet-F(ab)2 after incubation in PBS and 1% BSA over 24 h.
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