Research Article

Noninvasive Evaluation of EGFR Expression of Digestive Tumors Using 99mTc-MAG3-Cet-F(ab)2-Based SPECT/CT Imaging

Figure 5

Characterization of 99mTc-MAG3-Cet-F(ab)2 in vitro. (a) The radio-HPLC result of 99mTc-MAG3-Cet-F(ab)2. (b) Stability assay of 99mTc-MAG3-Cet-F(ab)2 in 1% BSA and NS. (c) Competition binding assay between 99mTc-MAG3-Cet-F(ab)2 and unlabeled cetuximab to MGC803 cells (d, e). Representative saturation binding curve of 99mTc-MAG3-Cet-F(ab)2 binding to MGC803 cells (d) and HT29 cells (e), indicating its higher affinity to MGC803 cells ( ligands/cell, ), compared to HT29 cells ( ligands/cell, ).
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