Celecoxib-Induced Modulation of Colon Cancer CD133 Expression Occurs through AKT Inhibition and Is Monitored by ⁸⁹Zr Immuno-PET

Figure 1

Deferoxamine conjugation and ⁸⁹Zr labeling of anti-CD133 IgG. (a) Diagram of ⁸⁹Zr-CD133 IgG (left), nonreduced SDS polyacrylamide gel electrophoresis (PAGE) of unmodified, TCEP-reduced, and DFO-conjugated anti-CD133 IgG (middle), and autoradiography of a native ⁸⁹Zr-CD133 IgG PAGE (right). (b) In vitro stability of ⁸⁹Zr-CD133 IgG assessed by radio-iTLC after incubation in PBS at 37°C for 96 hours (right).

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