Celecoxib-Induced Modulation of Colon Cancer CD133 Expression Occurs through AKT Inhibition and Is Monitored by ⁸⁹Zr Immuno-PET

Figure 6

Effects of celecoxib on HT29 tumor expression of CD133 and signaling pathways. (a) Immunoblots of cellular CD133, p-AKT, HIF-1α, and p-IκBα and nuclear p-NFκB. Tumor-bearing mice were intraperitoneally injected daily with vehicle or celecoxib for four consecutive days and were sacrificed on the day of the final treatment. (b) Quantified band intensities of CD133 and HIF-1α corrected by β-actin, p-AKT corrected by t-AKT, p-IκBα corrected by t-IκBα, and p-NFκB corrected by HDAC1. Bars are of %ID/g obtained from six animals per group. , ^†, and ^‡.

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