⁸⁹Zr Immuno-PET Imaging of Tumor PD-1 Reveals That PMA Upregulates Lymphoma PD-1 through NFκB and JNK Signaling

Figure 1

⁸⁹Zr-PD-1 IgG autoradiography, stability, and binding specificity. (a) Autoradiography of PD-10 column-eluted fractions of ⁸⁹Zr-PD-1 IgG on native PAGE. (b) In vitro radiochemical stability of ⁸⁹Zr-PD-1 IgG in PBS and FBS. (c) Complete blocking of ⁸⁹Zr-PD-1 IgG binding to EL4 cells by excess cold anti-PD-1 IgG demonstrates excellent target specificity. Bars represent the of triplicate samples per group. .

(a)

(b)

(c)