⁸⁹Zr Immuno-PET Imaging of Tumor PD-1 Reveals That PMA Upregulates Lymphoma PD-1 through NFκB and JNK Signaling

Figure 3

Effects of PMA stimulation on cultured EL4 cells. (a) Effects of 24 h treatment with graded doses of PMA on ⁸⁹Zr-PD-1 IgG binding (left) and PD-1 immunoblots with β-actin-corrected band intensities (right). (b) Effects of graded doses of PMA on Western blots of p-NFκB (left) and HIF-1α (right) with quantified protein band intensities that are corrected by controls. (c) Immunoblots of PD-1, HIF-1α, p-IκBα, and p-JNK with quantified protein band intensities corrected by controls at baseline and following 24 h treatment with PMA. All bars represent the of triplicate samples per group. , , , and .

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