Object-Place Recognition Learning Triggers Rapid Induction of Plasticity-Related Immediate Early Genes and Synaptic Proteins in the Rat Dentate Gyrus

Figure 3

Object recognition training increases Arc mRNA expression in the dentate gyrus. Arc mRNA in situ hybridization reveals sparse expression of Arc in both dorsal and ventral blades of the rat dentate gyrus of (a) CC, (b) C10, and (c) L10 animals. (d) Higher magnification shows typical Arc mRNA localization in the cell body and dendrites of granule cells. (e) Change in Arc-positive granule cell densities (relative to the CC group) in the dorsal and the ventral blade of the dentate gyrus across the C10, L10, C60, and L60 groups. Data are presented as mean ± SEM (𝑛=4 for all groups). Asterisks indicate 𝑃≤.05 (if not indicated otherwise, relative to CC group). Scale bars represent 200 𝜇m in (a)–(c) and 50 𝜇m in (d).

(a)

(b)

(c)

(d)

(e)