Deafferentation-Induced Redistribution of MMP-2, but Not of MMP-9, Depends on the Emergence of GAP-43 Positive Axons in the Adult Rat Cochlear Nucleus

Table 2

Antibodies.

Antigen	Immunogen	Source	Working dilution
Primary Antibodies
Growth associated protein 43 (GAP-43), clone 9-1E12	43-48 kDa GAP-43 (B-50, F1 or pp46) purified from rat brain.	Millipore, Temecula, Calif, USA; mouse, monoclonal, IgG1, cat.no. MAB347.	1 : 5,000
Matrix metalloproteinase 2 (MMP-2), clone H-76	raised against amino acids 1–76 of MMP-2 of human origin.	Santa Cruz Biotechnology, Inc. Santa Cruz, Calif, USA; rabbit, polyclonal, IgG, cat.no. sc-10736.	1 : 100
Matrix metalloproteinase 9 (MMP-9) clone C-20	raised against a peptide mapping at the C-terminus of MMP-9 of human origin.	Santa Cruz Biotechnology, Inc., Santa Cruz, Calif, USA; goat, polyclonal, IgG, cat.no. sc-6840.	1 :  250
Secondary Antibodies
Horse anti-mouse biotinylated	Mouse IgG	Vector laboratories	1 : 200
Goat anti-rabbit biotinylated	Rabbit IgG	Vector laboratories	1 : 200
Rabbit anti-goat biotinylated	Goat IgG	Vector laboratories	1 : 200