Drosophila Torsin Protein Regulates Motor Control and Stress Sensitivity and Forms a Complex with Fragile-X Mental Retardation Protein

Figure 6

2D analysis of proteins extracted from Drosophila adult heads. (a) Representative 2D electrophoresis image of protein extracts from control adult heads. A total of 6 spots were dysregulated in DTor-RNAi expressing fly brains. Blue arrows indicated spots with decreased volumes in DTor-RNAi expressing flies compared with controls. Red arrows point to spots with increased volumes in DTor-RNAi-expressing flies compared with controls. (b) Spots 483 and 580 in a control gel. (c) Spots 483 and 580 in a DTor-RNAi gel showing a comparatively larger volume. (d) Anti-ADH western blot analysis of genetic back ground and DTor-RNAi-expressing flies. (e) ADH protein levels detected from protein extracts from DTor-RNAi-expressing flies were 3.1-fold of those of control fly brains. <0.05.