Role of NADPH Oxidase-Induced Hypoxia-Induced Factor-1α Increase in Blood-Brain Barrier Disruption after 2-Hour Focal Ischemic Stroke in Rat

Figure 2

Effect of apocynin on occludin degradation and HIF-1α upregulation after 2 h MCAO. Western blot was recruited to detect occludin expression in nonischemic (NI) and ischemic (I) brain tissues. (a, b) Representative blots showed expression of occludin in regions of interest (ROI) 1 (a) and 2 (b) in apocynin- or vehicle-treated rats (upper panel). After the normalization, the relative band intensity of occludin to β-actin was quantified (lower panel). After two hours of MCAO, the occludin level in ROI 1 was significantly reduced (, compared with the vehicle group in ROI 1), while the occludin level in ROI 2 did not significantly decrease (). Apocynin treatment significantly alleviated the degradation of occludin (). (c, d) Representative blots demonstrated the expression of HIF-1α in ROI 1 (c) and ROI 2 (d) of apocynin- or vehicle-treated rats (upper panel). HIF-1α expression was quantified after normalization to β-actin (bottom panel). The expression of HIF-1α in ROI 1 () but not in ROI 2 () was significantly increased in the vehicle group after two hours of MCAO. Apocynin treatment significantly reduced the HIF-1α increase (). The data are expressed as the , per group.

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