Growth Hormone Increases BDNF and mTOR Expression in Specific Brain Regions after Photothrombotic Stroke in Mice

Figure 2

The effect of recombinant human growth factor (r-hGH) treatment poststroke on mammalian target of rapamycin (mTOR) in the peri-infarct region. (a) Representative blots of phosphorylated-mTOR (P-mTOR, active form), total-mTOR (T-mTOR), phosphorylated-p70S6-kinase P-p70S6K), and total-p70S6k (T-p70S6K). (b) T-mTOR and T-p70S6K levels were normalized to β-actin. P-mTOR and P-p70S6K levels were normalized to T-mTOR and T-p70S6K, respectively. Levels were expressed as a fold change (FC) of for each group relative to the mean of the Sham+Saline group (dotted line). We found a significant increase in T-mTOR protein expression within the peri-infarct region in stroked mice treated with r-hGH (blue squares) compared with saline (red circles). , 2-tailed t-test.

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