Research Article
Growth Hormone Increases BDNF and mTOR Expression in Specific Brain Regions after Photothrombotic Stroke in Mice
Table 1
List of antibodies used for western blot analyses.
| | Sources of antibodies | Dilution |
| BDNF | Santa Cruz, polyclonal rabbit anti-BDNF (precursor and mature), sc-546 | 1 : 5000 | T-mTOR | Cell Signalling, monoclonal mouse anti-total- mTOR, #4517 | 1 : 1000 | P-mTOR | Cell Signalling, monoclonal rabbit anti-phospho-mTOR (Ser2448), #5536 | 1 : 500 | T-p70S6K | Cell Signalling, polyclonal rabbit anti-total-p70S6K, #9202 | 1 : 1000 | P-p70S6K | Cell Signalling, monoclonal mouse anti-phospho-p70S6K, #9206 | 1 : 2000 | β-actin | Sigma-Aldrich, monoclonal anti-β-actin-HRP antibody, A3854 | 1 : 50000 | Rabbit IgG | Biorad, anti-rabbit-HRP antibody, #170-6515 | 1 : 7500 | Mouse IgG | Biorad, anti-mouse-HRP antibody, #170-6516 | 1 : 10000 |
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