Melatonin Ameliorates Ovarian Hyperstimulation Syndrome (OHSS) through SESN2 Regulated Antiapoptosis

Figure 4

The effect of melatonin on apoptosis related proteins in HUVECs and KGNs incubated with angiotensin II or H₂O₂. (a) The representative pictures of Western blotting of p-AMPK, AMPK, p-mTOR, mTOR and its downstream factors (p-S6K1, S6K1, p-4EBP1) as well as apoptosis related molecules (p17 caspase 3, caspase 3, BCL2) and GAPDH in melatonin and angiotensin II treated HUVECs; (b) the comparison of protein/GAPDH ratio in HUVECs; (c) the representative pictures of Western blotting of p-AMPK, AMPK, p-mTOR, mTOR and its downstream factors (p-S6K1, S6K1, p-4EBP1) as well as apoptosis related molecules (p17 caspase 3, caspase 3, Bcl2) and GAPDH in melatonin and H₂O₂ treated KGNs; (d) the comparison of protein/GAPDH ratio in KGNs. The results are expressed as mean ± SD. vs. control group; vs. control group; ^# vs. angiotensin II group; ^## vs. angiotensin II group. Ctrl: control; MLT: melatonin; Ang II: angiotensin II.

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