Antioxidant and Astroprotective Effects of a Pulicaria incisa Infusion

Figure 7

Peroxyl radical-induced oxidation of DCFH to DCF in primary astrocytes and the inhibition of oxidation by the Pi infusion.(a) Astrocytes were incubated for 1 h with Pi infusion. Then, the astrocytes were preloaded with DCF-DA for 30 min and washed. ABAP (0.6 mM) was added to the culture and the fluorescence intensity representing ROS levels was measured. (a) The fluorescence levels of cells that had been preincubation with 100 μg/mL Pi extract were measured at the indicated time points. ^∗∗∗𝑃<0.001 compared to cells treated with ABAP only. (b) The fluorescence levels of cells that had been pre-incubated with various concentrations of Pi extract were measured at the indicated time points. The results represent means ± SEM of three experiments (𝑛=12). ^∗𝑃<0.05,^∗∗𝑃<0.01,^∗∗∗𝑃<0.001.

(a)

(b)