Effects of Temperature and Water-Filtered Infrared-A Alone or in Combination on Healthy and Glyoxal-Stressed Fibroblast Cultures
Figure 1
Experimental conditions. (a) Transmission spectra of the Hydrosun 750 radiator with and without 750 nm cutoff filter. The reference spectrum was recorded from the same radiator without a water cuvette. Spectra are normalized to their maximal transmission. (b) A cell incubator was equipped with a wIRA radiator, whose radiation was guided by optical fibres into the incubator directly onto the cell culture plates (4 cm above the plates). The plates were placed in a large water bath (6 liters) to facilitate maintenance of constant temperatures during irradiation. (c) Cultures were allocated to four different treatment groups: group 1 control without wIRA, group 2 control with wIRA, group 3 glyoxal without wIRA, group 4 glyoxal with wIRA. Cells were either irradiated with wIRA or kept nonirradiated at the indicated temperatures and times after addition of glyoxal.
