TPEN Induces Apoptosis Independently of Zinc Chelator Activity in a Model of Acute Lymphoblastic Leukemia and Ex Vivo Acute Leukemia Cells through Oxidative Stress and Mitochondria Caspase-3- and AIF-Dependent Pathways
Figure 2
TPEN induces production of and H2O2 in a time-independent fashion. Jurkat cells (1 × 106 cells/mL) were either exposed to TPEN (3 μM) at 37°C for 1, 3, 6, 12, and 24 h or untreated for 24h (24U) (a) Representative light photomicrography illustrating formazan (FORMz) precipitation, as indicative of generation in Jurkat cells incubated with TPEN (3 μM) at 37°C for 3 h. (b) Representative histograms showing the percentage of DFC+ cells under TPEN (3 μM) exposure assessed at indicate interval of time, according to Material and Methods section (c) The percentage of positive FORMz and dichlorofluorescein (DCF) cells after each interval are expressed as a mean percentage ± S.D. from three independent experiments.
