TPEN Induces Apoptosis Independently of Zinc Chelator Activity in a Model of Acute Lymphoblastic Leukemia and Ex Vivo Acute Leukemia Cells through Oxidative Stress and Mitochondria Caspase-3- and AIF-Dependent Pathways
Figure 5
Effect of glucose on Jurkat cells exposed to TPEN. (a) The percentage of positive AO/EB/Hoechst staining of Jurkat cells (1 × 106 cells/mL) incubated under glucose-starvation (GS), glucose 11 mM (G11), or glucose 55 mM (G55) medium in absence or presence of TPEN (3 μM) at 37°C for 1, 3, 6, 12, and 24 h with normal, stage I, and stage II nuclei morphology are expressed as a mean percentage ± S.D. from three independent experiments. (b) Representative density (dot) plots showing PI/DiOC6(3) percentage of four different cell populations at 12 h of incubation in GS, G11, and G55 medium with or without TPEN (3 μM), as described in Material and Methods section. (c) Representative histograms showing SubG1 cell populations at 12 hours in GS, G11, and G55 medium with or without TPEN (3 μM), as described in according to Material and Methods section.
