Research Article
Isoliquiritigenin-Induced Differentiation in Mouse Melanoma B16F0 Cell Line
Table 1
Primer sequences used.
| Gene | Primer | Annealing temperature/°C | Cycles |
| TYR | (+) 5′-GGCCAG CTT TCA GGC AGA GGT-3′ (−) 5′-TGG TGC TTC ATG GGC AAA ATC-3′ | 60 | 32 | TRP-1 | (+) 5′-GCT GCA GGA GCC TTC TTT CTC-3′ (−) 5′-AAG ACG CTG CAC TGC TGG TCT-3′ | 61 | 30 | TRP-2 | (+) 5′-GGA TGA CCG TGA GCAATG GCC-3′ (−) 5′-CGG TTG TGA CCAATG GGT GCC-3′ | 63 | 30 | GAPDH | (+) 5′-CAA GGT CAT CCA TGA CAA CTT-3′ (−) 5′-GTC CAC CAC CCT GTT GCT GTA-3′ | 58 | 28 |
|
|