Desferrioxamine Attenuates Doxorubicin-Induced Acute Cardiotoxicity through TFG-𝜷/Smad p53 Pathway in Rat Model

Table 1

Primers and probes sequence for TGF-β, Smad2, Smad4, Smad7, CDKN2A, Mdm2, and p53.

Gene	Forward primer	Reverse primer	Probe
TGF-β	5′-TGCCTGACGGTCAGGTCA-3′	5′-CAGGAAGGAAGGCTGGAG-3′	FAM-CACTATCGGCAATGAGCGGTTCCG-TAMRA
Smad7	5′-CCCCATCACCTTAGTCGACTCT-3′	5′-GACAGTCTGCAGTTGGTTTGAGA-3′	VIC-CCCCTCCTCCTTACTCCAGATACCCGA-TAMRA
Smad4	5′-ACCAACTTCCCCAACATTCCT-3′	5′- ACTATGGCTGCCTGCCAGAA-3′	FAM-TGGCTTCCACAAGTCAGCCGGC-TAMRA
Smad2	5′-CCATCCCCGAGAACACTAACTT-3′	5′-TGGTGGTCGCTAGTTTCTCCAT-3′	VIC-AACCCCAGAGCAATATTCCAGAAACCCC-TAMRA
CDKN2A	5′-GGGTCACCGACAGGCATAAC-3′	5′-AGTCTCGCGTTGCCAGAAGT-3′	FAM-TGCTCAAGCACGCCCAGGTGC-TAMRA
Mdm2	5′-GGTTGTGGGCTGCAGAGAAG-3′	5′-TGCAAGGATCAAATGTGTGTCA-3′	HEX-TCAGCCGGTAAAGGCGCCCACT-TAMRA,
p53	5′-CACCATGAGCGTTGCTCTGAT-3′	5′-GATTTCCTTCCACCCGGATAA-3′	FAM-ACGGCCTGGCTCCTCCCCAAC-TAMRA
β-actin	5′-TGCCTGACGGTCAGGTCA-3′	5′-CAGGAAGGAAGGCTGGAAG-3′	HEX-CACTATCGGCAATGAGCGGTTCCG-TAMRA