The Critical Role of Redox Homeostasis in Shikonin-Induced HL-60 Cell Differentiation via Unique Modulation of the Nrf2/ARE Pathway

Table 1

Primer sequences used.

Gene	Primers	Tm (°C)
CD14 [15]	sense: 5′-AAAGGACTGCCAGCCAAGCT-3′	58
	anti-sense: 5′-GATTCCCGTCCAGTGTCAGGT-3′
CD11b [12]	sense: 5′-CAGAGCGTGGTCCAGCTTCA-3′	58
	anti-sense: 3′-CCTTCATCCGCCGAAAGTCA-5′
NQO1 [16]	sense: 5′-GAGGACCTCCTTCAACTATGCC-3′	59
	anti-sense: 5′-CCTTTGTCATACATGGCAGCG-3′
GCLC [17]	sense: 5′-ATGATGCCAACGAGTCTGAC-3′	57
	anti-sense: 5′- CGCCTTTGCAGATGTCTTTC-3′
GCLM [17]	sense: 5′-AGGAGCTTCGGGACTGTATT-3′	57
	anti-sense: 5′-TGGGCTTCAATGTCAGGGAT-3′
GST [18]	sense: 5′-ATGGGCCTAGAGCTGTTTCT-3′	57
	anti-sense: 5′-AGCCCAGGTACTCATGAACA-3′
GPX [18]	sense: 5′-GGGGCCTGGTGGTGCTCGGCT-3′	65
	anti-sense: 5′- CAATGGTCTGGAAGCGGCGGC-3′
CAT [17]	sense: 5′-AACTGGGATCTTGTGGGAA-3′	55
	anti-sense: 5′-GACAGTTCACAGGTATCTG-3′
GR [18]	sense: 5′-GAAAAGGCTGTAATTTTATTTTCAA-3′	55
	anti-sense: 5′-ATCAAAAGTCCCTTCCTCTGC-3′
GAPDH	sense: 5′-CAAGGTCATCCATGACAACTTTG-3′	57
	anti-sense: 5′-GTCCACCACCCTGTTGCTGTAG-3′