Dietary Flavonoids as Therapeutics for Preterm Birth: Luteolin and Kaempferol Suppress Inflammation in Human Gestational Tissues In Vitro
Figure 2
Effect of luteolin and kaempferol on the COX-prostaglandin pathway. ((a), (b)) Fetal membranes were incubated with 10 μg/mL LPS in the absence or presence of 20 μM luteolin or 100 μM kaempferol for 24 h ( patients). (a) COX-2 mRNA expression was quantified by qRT-PCR. (b) PGE2 and PGF2α concentrations in the conditioned media were assayed using EIA. Each bar represents the mean ± SEM. versus basal (one-way ANOVA). ((c), (d)) Primary myometrial cells were incubated with 0.5 ng/mL IL-1β in the absence or presence of 20 μM luteolin or 100 μM kaempferol for 24 h ( patients). (c) COX-2 mRNA expression was quantified by qRT-PCR. (d) PGE2 and PGF2α concentrations in the conditioned media were assayed using EIA. Each bar represents the mean ± SEM. versus basal (one-way ANOVA).
