Enhanced Prostacyclin Synthesis by Adenoviral Gene Transfer Reduced Glial Activation and Ameliorated Dopaminergic Dysfunction in Hemiparkinsonian Rats
Figure 6
Nigral Ad-COX-1/PGIS infusion ameliorated 6-OHDA-induced dopamine neuronal loss and behavioral deficit. (a)(c) Representative micrographs of TH immunoreactivity in rat substantia nigra (SNpc) of each group. 20 m thick coronal brain sections, In (a) 6-OHDA-lesioned brain section, (b) 6-OHDA-lesioned + AdGFP-transduced brain section and (c) 6-OHDA-lesioned + AdCOX1/PGIS-transduced brain section. (d) Apomorphine-induced rotational behaviors in 6-OHDA-treated rats. TH-positive neurons in (c) were rescued by AdV-COX1/PGIS adenoviral gene transfer. 6-OHDA treatment severely depletes TH-positive neurons in right SN compared with untreated left site. Adenoviral vectors were injected into SN within 30 min after 6-OHDA treatment. Treatment rescued TH-positive neurons ipsilateral to gene delivery. In all cases, Ad treatment had no effect on the contralateral SNpc. Data are means SEM from 8, 5, and 13 rats for Lesion, Lesion + AdGFP, and Lesion + AdCOX-1/PGIS groups, respectively. compared to lesion only at the same time.
