Atorvastatin Represses the Angiotensin 2-Induced Oxidative Stress and Inflammatory Response in Dendritic Cells via the PI3K/Akt/Nrf 2 Pathway

Figure 1

Effects of atorvastatin on ROS formation (a), SOD activity (b), and MDA levels (c) in BMDCs in the presence of Ang 2. BMDCs were incubated with Ang 2 (100 nM) alone or in combination with various concentrations (2.5, 5, or 10 μM) of atorvastatin for 24 h. (a) The intracellular ROS levels were measured via flow cytometry using DCFH-DA. (b) The SOD activity and (c) MDA levels were measured at 450 and 532 nm, respectively, using ELISA kits. The data are shown as the mean ± (SD) (𝑛=3); ^∗𝑃<0.05 versus control; ^#𝑃<0.05 versus Ang 2 group; ^$𝑃<0.05 versus Ang 2 + 2.5 μM atorvastatin group; ^&𝑃<0.05 versus Ang 2 + 5 μM atorvastatin group.

(a)

(b)

(c)