Leptin Level and Oxidative Stress Contribute to Obesity-Induced Low Testosterone in Murine Testicular Tissue

Figure 5

The figure showed the TUNEL apoptosis assay in Leydig cells after 8-week and 19-week high-fat diet. Photographs of control at 8 weeks (a), DIO group at 8 weeks (b), DIO-R group at 8 weeks (c), control group at 19 weeks (d), DIO group at 19 weeks (e), and DIO-R group (f) at 19 weeks were in this figure. The sections were stained with DAB, and the magnification was set at ×40. Data are mean ± SD for six animals in each group. The effects of 8-week and 19-week high-fat diet on Leydig cells apoptotic rate (g) were also shown in this figure. ^∗∗𝑃<0.01 denotes statistical significance compared with control-8w group; ^##𝑃<0.01 denotes statistical significance compared with control-19w group.

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