Flutamide-Induced Cytotoxicity and Oxidative Stress in an In Vitro Rat Hepatocyte System

Figure 5

FLU-induced GSH depletion (measured at 30 min). Refer to the Materials and Methods section for the description of the experiments performed and experimental conditions. GSH: reduced glutathione; G: glucose; GO: glucose oxidase; HRP: horseradish peroxidase; PTU: 6-N-propyl-2-thiouracil; NAC: N-acetylcysteine; Trolox: (±)-6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; resveratrol: 3,5,4′-trihydroxy-trans-stilbene; TEMPOL: 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl; DPPD: N,N′-diphenyl-p-phenylenediamine.Significant compared to control (only hepatocytes). ^#Significant compared to 75 µM FLU. Significant compared to 75 µM FLU + H₂O₂ generating system + PTU.