Dietary Blueberry and Bifidobacteria Attenuate Nonalcoholic Fatty Liver Disease in Rats by Affecting SIRT1-Mediated Signaling Pathway

Figure 6

Immunohistochemistry (IHC) analysis for the effect of blueberry juice and bifidobacteria on PPAR-α expression in NAFLD rat livers (200x). CG: in control group, PPAR-α was highly expressed in cytoplasm with brown color. MG: in NAFLD model group, PPAR-α was lowly expressed in cytoplasm with very light brown color. SI: in NAFLD model group with SIRT1 RNAi, PPAR-α was lowly expressed in cytoplasm with very light brown color. BJ: in blueberry juice group, PPAR-α was highly expressed in cytoplasm with brown color. BJSI: in blueberry juice and SIRT1 RNAi group, the expression of PPAR-α was significantly inhibited with very light brown color. BJB: in blueberry juice and bifidobacteria group, PPAR-α was highly expressed in cytoplasm with brown color. BJBSI: in blueberry juice and bifidobacteria and SIRT1 RNAi group, the expression of PPAR-α was completely inhibited with blue color.