Lentivirus-Mediated Nox4 shRNA Invasion and Angiogenesis and Enhances Radiosensitivity in Human Glioblastoma
Figure 1
Verification of knockdown of Nox4 expression in U87MG and U251 cells by lentivirus-mediated RNA interference. (a) The expression levels of Nox4 mRNA were measured by qRT-PCR. There was a dramatic decrease of Nox4 mRNA in the Nox4 shRNA group (). No significant difference was observed between noninfected cells and scrambled control. (b) The expression levels of Nox4 protein were measured by Western blot (line 1, noninfected; line 2, scrambled control; line 3, Nox4-shRNA). The protein levels in the Nox4-shRNA group decreased significantly compared to scrambled and noninfected cells. The GAPDH gene and the β-actin protein are the internal controls for qRT-PCR and Western blot analysis, respectively. Columns, mean; bars, SD; *, versus noninfected and scrambled control; #, versus noninfected.
