The Beneficial Effect of Melatonin in Brain Endothelial Cells against Oxygen-Glucose Deprivation Followed by Reperfusion-Induced Injury
Figure 2
Immunocytochemistry to measure ROS generation in bEND.3 cells after OGD/R-induced injury. bEND.3 cells were treated with melatonin for 24 h before OGD/R injury. ROS levels were measured using DCF-DA. (a) ROS levels in only melatonin treatment groups (both 10 nM and 100 nM melatonin pretreatment groups) were the same as the normal control group. ROS levels in bEND.3 cells were increased in OGD/R injury exposed group. Under OGD/R injury, ROS levels in the melatonin pretreatment group were decreased compared to OGD/R injury exposed group. Melatonin decreased the OGD/R-induced increase in DCF-DA-positive cells (green). (b) ROS production was calculated by measuring the intensity of ROS. This graph shows relative intensity as a percentage of OGD/R injury exposed group. Data are expressed as mean ± S.E.M. (# and *). 2′,7′-Dichlorodihydrofluorescein diacetate (DCF-DA): green. Scale bar = 400 µm.
